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Image Search Results
Journal: medRxiv
Article Title: Clinical validation of a novel metagenomic nanopore sequencing method for detecting viral respiratory pathogens
doi: 10.64898/2026.02.06.26345651
Figure Lengend Snippet: Red indicates passed and black failed. RNA from murine respirovirus, orthoreovirus and Zika virus were spiked as cDNA synthesis and SISPA controls, MS2 phage was spiked as an extraction, cDNA synthesis and SISPA control. Within the derivation set MS2 was not required to pass QC due to poor performance of the laboratory MS2 stock; in both validation and derivation only detection of 2/3 virus spikes was required to pass QC.
Article Snippet: Extracted RNA (8μl) was treated with DNAse, as recommended, then spiked with three reverse transcription/amplification controls (1μl each, containing approximately 10 4 genome copies each of Zika virus (ATCC-VR-1838DQ),
Techniques: Virus, cDNA Synthesis, Extraction, Control, Biomarker Discovery